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NGS · Data Science · Microbial Ecology

Shan Thomas

Molecular biologist and data scientist with 10+ years of experience in next-generation sequencing, bioinformatics, and microbial ecology research. Currently pursuing a PhD at the University of Toledo.

Areas of Specialization

A decade of experience across three complementary domains — integrating laboratory science, computational analysis, and ecological research.

Domain 01

NGS Scientist

End-to-end Next-Generation Sequencing expertise from library prep to data delivery. Experienced with Illumina and Nanopore platforms for amplicon, WES, and WGS workflows.

Illumina Nanopore Library Prep Sanger QC LIMS
10 Years
5000+ Libraries
Domain 02

Data Scientist

Statistical analysis and machine learning for biological data interpretation. Microbiome analysis, diversity metrics, and predictive modeling on HPC systems.

R Python Markdown ML HPC Shiny Cloud Computing Nextflow
1 R Package
4 Web Apps
Domain 03

Microbial Ecologist

Exploring microbial communities through metabarcoding and ancient DNA analysis. From soil microbiomes to paleoenvironmental reconstructions.

Metabarcoding 16S rRNA 18S rRNA QIIME2 DADA2 Ancient DNA qPCR
6 Publications
100+ Citations

Technical Skills

Laboratory & Sequencing

Illumina (MiSeq, NextSeq, NovaSeq) Oxford Nanopore (MinION) MGI Platforms Sanger Sequencing Library Preparation DNA/RNA Extraction PCR & qPCR Quality Control

Computational & Data

R / R Shiny Python QIIME2 / DADA2 Nextflow / Snakemake HPC / SLURM Docker Git / GitHub AWS / GCP

Analysis & Research

Metabarcoding (16S, 18S, ITS) Amplicon / WES / WGS Microbial Community Analysis Ancient DNA (PalEnDNA) Statistical Modeling Data Visualization Machine Learning LIMS Management

Technologies & Platforms

Illumina
Nanopore
Nextflow
Shiny
SLURM

Featured Projects

Computational tools and analysis pipelines for NGS and microbiome research.

Guides & Tutorials

Metabarcoding Guide

Comprehensive tutorial for processing metabarcoding datasets on HPC systems.

Documentation Tutorial HPC

Markdown & Zotero

Workflow for scientific writing using Markdown with Zotero integration.

Documentation Writing

Git & GitHub Guide

Beginner's guide to version control with Git and GitHub for research.

Tutorial Git

Experience

A decade of bridging laboratory science and computational analysis.

2022 - Present Current

PhD in Biology

University of Toledo
Ohio, USA
  • Microbial ecology research using metabarcoding and machine learning approaches
  • High-performance computing workflows using SLURM scheduling systems
Research HPC ML
2020 - 2021

Technical Sales Specialist

Specialized Scientific Solutions
Riyadh, Saudi Arabia (Remote)
  • Provided technical consultation for MGI and Illumina NGS platforms
  • End-user training and workflow optimization support
Sales Training NGS
2020

Assistant Manager, Molecular Biology

LifeCell International
Chennai, India
  • Laboratory management and team coordination
  • Amplicon, WES, and WGS library preparation workflows
Management NGS Lab
2017 - 2020

Research Associate

MedGenome Labs
Bangalore, India
  • Sanger and NGS sequencing operations
  • Illumina instrument maintenance and troubleshooting
  • LIMS management and workflow optimization
Sequencing LIMS QC
2015 - 2017

Researcher

Geobiotechnology Lab
Trichy, India
  • Soil microbial ecology and ancient DNA metabarcoding
Ecology Ancient DNA Microbiome
2013 - 2015

Senior Research Fellow

Indian Agricultural Research Institute - ITCC
New Delhi, India
  • Microbiome analysis and fungal molecular taxonomy
  • Entomology metabarcoding projects
Taxonomy Fungi Entomology

Publications

Peer-reviewed contributions to microbial ecology and ancient DNA research.

Thomas, S. P., Shanmuganathan, B., Krishnan, S., et al. (2018). Metabarcoding of PalEnDNA as an efficient tool to recover ancient bacterial diversity. Geomicrobiology Journal, 35(9), 798-803.

Thomas, S. P., Shanmuganathan, B., Jaiswal, M. K., et al. (2019). Legacy of a Pleistocene bacterial community: Patterns in community dynamics through changing ecosystems. Microbiological Research, 226, 65-73.

Stetten, L., Boyanov, M. I., O'Loughlin, E. J., Thomas, S. P., et al. (2025). Biogeochemical controls on iron speciation and cycling across upland to shoreline gradients in freshwater and estuarine coastal soils. Science of The Total Environment.

View All Publications

Get in Touch

Interested in collaboration or have an opportunity? Let's connect.

Collaboration & Opportunities

I bring 10+ years of hands-on NGS experience — from amplicon library prep to Illumina/MGI troubleshooting — combined with computational skills in bioinformatics and data visualization.

Available for Field Application Scientist and Technical Support roles, NGS data analysis consulting, and R/Shiny training workshops.

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