Profile Work Experience Publications Contact

NGS · Bioinformatics · Microbial ecology

Shan
Thomas

NGS scientist and computational biologist. I turn samples into sequence, and sequence into insight — ten years across the bench and the pipeline, from Illumina library prep to reproducible cloud workflows.

Where the bench meets the pipeline

Most people in sequencing sit on one side of the glass — either the wet lab or the analysis. I've spent a decade on both. That means I design experiments knowing how the data will be processed, and I build pipelines knowing exactly how the reads were made.

My work runs from amplicon, exome, and whole-genome library prep on Illumina, MGI, and Nanopore platforms through to metabarcoding, diversity analysis, and machine learning on HPC and cloud infrastructure — now focused on microbial ecology as a PhD student at the University of Toledo.

Experience
10 years
Worked across
3 countries
Libraries prepared
5,000+
Peer-reviewed papers
6

At the bench

  • Illumina — MiSeq, NextSeq, NovaSeq
  • MGI & Oxford Nanopore (MinION)
  • Amplicon, WES & WGS library prep
  • Sanger sequencing & PCR / qPCR
  • DNA / RNA extraction & QC
  • LIMS & instrument maintenance

In the pipeline

  • R / R Shiny & Python
  • Nextflow & Snakemake
  • QIIME2 / DADA2
  • HPC / SLURM & Docker
  • AWS & Google Cloud
  • Git & reproducible workflows

Across the study

  • Metabarcoding — 16S, 18S, ITS
  • Microbial community analysis
  • Ancient DNA (PalEnDNA)
  • Statistical modeling & ML
  • Data visualization
  • Study design & delivery

Tools I've built and shipped

R Shiny application R · Shiny · Visualization

MicrobeStudio

An interactive workbench for metabarcoding data — diversity metrics, ordination, and taxa distribution without writing a line of code. Built for researchers who need results, not a console.

Open the app
WebR learning platform webR · R · Education

BioRLab

A browser-based R classroom for biology undergraduates. Runs R entirely in the browser through webR — no installs, no setup — so students learn to code with their own data from the first session.

Open the platform
Cloud deployment R · Docker · Google Cloud Run

Biodiversity Dashboard

A containerized biodiversity explorer over GBIF occurrence data, served from Google Cloud Run — from R script to a scalable hosted service.

Open the app
R package R · API · LLM tooling

CodeSparkR

Brings 400+ language models into the R console through OpenRouter, so analysts can get coding assistance without leaving their environment.

View on GitHub
Streamlit application Python · Streamlit · NLP

ProtoCart

A lab assistant that reads a protocol, extracts the materials it calls for, and assembles a vendor-linked ordering list — turning a chore into a click.

Open the app
Cloud infrastructure AWS · EC2 · S3 · IAM

Metabarcoding on AWS

A reference implementation for running metabarcoding workflows on AWS — compute, storage, and access control provisioned for scalable bioinformatics.

View on GitHub

Guides & documentation

A decade across labs and countries

2022 — Present Current

PhD Student, Biology

University of Toledo · Ohio, USA

Microbial ecology research combining metabarcoding with machine learning, run on SLURM-scheduled HPC infrastructure.

2020 — 2021

Technical Sales Specialist

Specialized Scientific Solutions · Riyadh, Saudi Arabia (Remote)

Technical consultation, workflow optimization, and end-user training across MGI and Illumina NGS platforms.

2020

Assistant Manager, Molecular Biology

LifeCell International · Chennai, India

Led amplicon, WES, and WGS library preparation and coordinated a production sequencing team.

2017 — 2020

Research Associate

MedGenome Labs · Bangalore, India

Ran Sanger and NGS operations, maintained and troubleshot Illumina instruments, and managed LIMS workflows.

2015 — 2017

Researcher

Geobiotechnology Lab · Trichy, India

Soil microbial ecology and ancient-DNA metabarcoding, including published work on Pleistocene bacterial communities.

2013 — 2015

Senior Research Fellow

Indian Agricultural Research Institute — ITCC · New Delhi, India

Microbiome analysis, fungal molecular taxonomy, and entomology metabarcoding projects.

Peer-reviewed research

  1. Thomas, S. P., Shanmuganathan, B., Krishnan, S., et al. (2018). Metabarcoding of PalEnDNA as an efficient tool to recover ancient bacterial diversity. Geomicrobiology Journal, 35(9), 798–803. DOI ↗
  2. Thomas, S. P., Shanmuganathan, B., Jaiswal, M. K., et al. (2019). Legacy of a Pleistocene bacterial community: patterns in community dynamics through changing ecosystems. Microbiological Research, 226, 65–73. DOI ↗
  3. Stetten, L., Boyanov, M. I., O'Loughlin, E. J., Thomas, S. P., et al. (2025). Biogeochemical controls on iron speciation and cycling across upland to shoreline gradients in freshwater and estuarine coastal soils. Science of The Total Environment. Link ↗

Let's work together

I'm open to Field Application Scientist and NGS roles, computational biology positions, data-analysis consulting, and R / Shiny training — as well as research collaborations.

Send a note and I'll get back to you.