Shan Thomas

Molecular Biologist | NGS Specialist | Data Scientist

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Shan Thomas

About Me

A dedicated professional with expertise in Next-Generation Sequencing (NGS) and microbial ecology.

My Expertise

With hands-on experience across the full NGS workflow—from precise library preparation and sequencing (Illumina, Nanopore) to data analysis using SLURM pipelines and R—I approach each step with a methodical, problem-solving mindset.

My focus is on making molecular insights accurate, reproducible, and meaningful. Whether troubleshooting workflows or optimizing protocols, I aim to bring clarity and efficiency to every stage of the process.

NGS Techniques

End-to-end sequencing solutions.

Microbial Ecology

Insights into microbial communities.

Bioinformatics

Decoding complex biological data.

Workflow Automation

Streamlined research processes.

Skills Snapshot

Wet Lab Skills

Nucleic Acid Isolation
100%
PCR
95%
NGS
85%
Sanger Sequencing
100%

Dry Lab Skills

Primer/gBlock Designing
90%
Variant Analysis-Sanger
90%
QIIME2
75%
DADA2
80%

Programming & Data Analysis Languages

R
85%
HTML
70%
Shell
65%
CSS
60%

Career Journey

A timeline of my professional and educational milestones.

PhD in Biology

University of Toledo | 2022–Present

Ohio, USA

  • Microbial ecology, metabarcoding, ML
  • SLURM / HPC

Technical Sales Specialist

Specialized Scientific Solutions | 2020–2021

Riyadh, Saudi Arabia (Remote)

  • MGI, Illumina: Technical Sales
  • Customer Support

Assistant Manager, Molecular Biology

LifeCell International | 2020

Chennai, India

  • Lab management
  • Workflow automation
  • Amplicon, WES, WGS: Library Prep

Research Associate

MedGenome Labs | 2017–2020

Bangalore, India

  • DNA sequencing: Sanger, NGS
  • Illumina instrument: handling and maintenance
  • PCR/qPCR
  • LIMS, Workflow optimization

Researcher

Geobiotechnology Lab | 2015–2017

Trichy, India

  • Soil microbial ecology, aDNA Metabarcoding
  • DNA extraction, PCR

Senior Research Fellow

Indian Agricultural Research Institute–ITCC | 2013–2015

New Delhi, India

  • Microbiome analysis at Indian Type Culture Collection
  • PCR, Barcoding, Fungal Molecular Taxonomy
  • Entomology: Metabarcoding

MSc Biotechnology

Bharathidasan University

Trichy, India

Projects & Publications

Explore some of the work I've done and contributions to scientific literature.

Projects

Microbiome Dashboard

A Shiny dashboard to explore diversity, ordination, and taxa distribution from metabarcoding data.

Introduction to Metabarcoding

A beginner-friendly guide to processing metabarcoding datasets on high-performance computing systems.

Writing using Markdown and Zotero

A workflow to write and cite scientific articles using Markdown with Zotero integration for reference management.

Beginners guide to Git and GitHub

A quick start guide to Git and GitHub, including step-by-step instructions to integrate version control into RStudio.

Publications

  • Thomas, S. P., Shanmuganathan, B., Krishnan, S., Goswami, K., Dev, M., Jaiswal, M. K., Kumaresan, A., & Sadasivam, S. K. (2018). Metabarcoding of PalEnDNA as an efficient tool to recover ancient bacterial diversity. Geomicrobiology Journal, 35(9), 798–803. https://doi.org/10.1080/01490451.2018.1474507

  • Thomas, S. P., Shanmuganathan, B., Jaiswal, M. K., Kumaresan, A., & Sadasivam, S. K. (2019). Legacy of a Pleistocene bacterial community: Patterns in community dynamics through changing ecosystems. Microbiological Research, 226, 65–73. https://doi.org/10.1016/j.micres.2019.06.001

  • Machado-Silva, F., Weintraub, M., Ward, N. D., Doro, K. O., Regier, P. J., Ehosioke, S., Thomas, S. P., Peixoto, R. B., … Myers-Pigg, A. N. (2023). Groundwater redox dynamics across the terrestrial-aquatic interface of Lake Erie coastal ecosystems. bioRxiv. https://doi.org/10.1101/2023.06.12.544684

  • Ehosioke, S. Adebayo, M. B., Bailey, V. L., Peixoto, R. B., Emmanuel, E. D., Machado-Silva, F., Regier, P. J., Spanbauer, T., Thomas, S. P., Ward, N. D., Weintraub, M. N., & Doro, K. O. (2024). Geophysical methods reveal the soil architecture and subsurface stratigraphic heterogeneities across land-lake interfaces along Lake Erie. Journal of Soils and Sediments, 24, 2215–2236. https://doi.org/10.1007/s11368-024-03787-w

  • Kamil, D., Prameeladevi, T., Ganesh, S., Prabhakaran, N., Nareshkumar, R., & Thomas, S. P. (2017). Green synthesis of silver nanoparticles by entomopathogenic fungus Beauveria bassiana and their bioefficacy against mustard aphid (Lipaphis erysimi Kalt.). Indian Journal of Experimental Biology, 55(8), 555–561. ResearchGate link

Get in Touch

I'm open to opportunities and collaborations in the field of Next-Generation Sequencing (NGS) and beyond.

Contact Me

I am actively seeking opportunities as a Field Application Scientist or in Technical Support within the Next-Generation Sequencing (NGS) sector. If you have an opening—or would like to explore how I can contribute to your team—please feel free to get in touch.

Additionally, I offer professional NGS data analysis services and hands-on Basic R workshops, tailored to suit research labs, academic institutions, or individual learning goals.

Send a Message

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